3D ReconstructionStack of tissue images ready for reconstruction

The figures show data from the same piece of tissue, which is a block of approximately 6 x 6 x 7.5 microns of neuropil in mouse sensorimotor cortex, layer 5. The tissue was sliced on ATUM, using a slice thickness of just below 30 nm. Each slice was imaged in an electron microscope (EM) at a resolution of 3 nm per pixel. Images were digitally aligned and this block was cropped out from the resulting image stack.

This image shows the stack of raw electron-microscopic images. Cell membranes and organelles are clearly visible.

Credit: Daniel Berger